ARG81445
Human CXCL13 / BCA1 ELISA Kit
Human CXCL13 / BCA1 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81445-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81445-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81445-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81445-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81445-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81445-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81445-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81445-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81445-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81445-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81445-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81445 Human CXCL13 / BCA1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CXCL13 / BCA1 in serum, plasma (heparin, EDTA), saliva and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | CXCL13 / BCA1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 7.8 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA), saliva and cell culture supernatants. |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.8% Inter-Assay CV: 7.7% |
| Alternate Names | BCA-1; Angie; C-X-C motif chemokine 13; CXC chemokine BLC; ANGIE2; B lymphocyte chemoattractant; B cell-attracting chemokine 1; BLR1L; Small-inducible cytokine B13; SCYB13; ANGIE; BLC; BCA1 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CXCL13 |
| Gene Full Name | chemokine (C-X-C motif) ligand 13 |
| Background | B lymphocyte chemoattractant, independently cloned and named Angie, is an antimicrobial peptide and CXC chemokine strongly expressed in the follicles of the spleen, lymph nodes, and Peyer's patches. It preferentially promotes the migration of B lymphocytes (compared to T cells and macrophages), apparently by stimulating calcium influx into, and chemotaxis of, cells expressing Burkitt's lymphoma receptor 1 (BLR-1). It may therefore function in the homing of B lymphocytes to follicles. [provided by RefSeq, Oct 2014] |
| Function | Chemotactic for B-lymphocytes but not for T-lymphocytes, monocytes and neutrophils. Does not induce calcium release in B-lymphocytes. Binds to BLR1/CXCR5. [UniProt] |
| Highlight | Related products: CXCL antibodies; CXCL ELISA Kits; CXCL Duos / Panels; CXCL recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81445 Human CXCL13 / BCA1 ELISA Kit standard curve image
ARG81445 Human CXCL13 / BCA1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81445 Human CXCL13 / BCA1 ELISA Kit User's manual |
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