ARG80121
Human CXCL12 / SDF1 alpha ELISA Kit
Human CXCL12 / SDF1 alpha ELISA Kit for ELISA and Human
Cell Biology and Cellular Response kit; Immune System kit; Microbiology and Infectious Disease kit
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG80121-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80121-002 | Standard (Lyophilized) | 3 X 4 ng/vial | 4°C |
| ARG80121-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG80121-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80121-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80121-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80121-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80121-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80121-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80121-010 | STOP solution | 12ml | 4°C |
| ARG80121-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG80121 Human CXCL12 / SDF1 alpha ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CXCL12 / SDF1 alpha in serum, plasma and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | No significant cross-reactivity or interference with Human beta-ECGF, GRO alpha, GRO beta, GRO gamma, MCP-1, MIP-1 alpha, MIP-1 beta, PTN, RANTES；mouse MIP-1 alpha, MIP-1 beta. The cross-reactivity tested with 50 ng/ml of Human SDF-1 beta is <10% and rat SDF-1 beta is <1%. |
| Target Name | CXCL12 / SDF1 alpha |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 62.5 pg/ml |
| Sample Type | Serum, plasma and cell culture supernatants. |
| Standard Range | 125 - 8000 pg/ml |
| Sample Volume | 100 µl |
| Precision | CV: <10% |
| Alternate Names | TPAR1; SDF1; C-X-C motif chemokine 12; Pre-B cell growth-stimulating factor; TLSF; PBSF; SDF-1; Intercrine reduced in hepatomas; IRH; hSDF-1; 3-72; SCYB12; hIRH; 3-67; Stromal cell-derived factor 1 |
Application Instructions
| Assay Time | 4 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CXCL12 |
| Gene Full Name | chemokine (C-X-C motif) ligand 12 |
| Background | Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation. [provide by uniprot] |
| Highlight | Related products: CXCL12 antibodies; CXCL12 ELISA Kits; CXCL12 recombinant proteins; Related news: LKB1 deficiency in T cells promotes gut tumors New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Cell Biology and Cellular Response kit; Immune System kit; Microbiology and Infectious Disease kit |
| PTM | Processed forms SDF-1-beta(3-72) and SDF-1-alpha(3-67) are produced after secretion by proteolytic cleavage of isoforms Beta and Alpha, respectively. The N-terminal processing is probably achieved by DPP4. Isoform Alpha is first cleaved at the C-terminus to yield a SDF-1-alpha(1-67) intermediate before being processed at the N-terminus. The C-terminal processing of isoform Alpha is reduced by binding to heparin and, probably, cell surface proteoglycans. |
Images (1) Click the Picture to Zoom In
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ARG80121 Human CXCL12 / SDF1 ELISA Kit standard curve image
ARG80121 Human CXCL12 / SDF1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG80121 Human CXCL12 / SDF1 alpha ELISA Kit User manual |
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