ARG80128
Human CCL2 / MCP1 ELISA Kit
Human CCL2 / MCP1 ELISA Kit for ELISA and Human
Cancer kit; Cell Biology and Cellular Response kit; Immune System kit; Metabolism kit
Publication3
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG80128-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80128-002 | Standard (Lyophilized) | 3 X 1 ng/vial | 4°C |
| ARG80128-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG80128-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80128-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80128-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80128-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80128-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80128-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80128-010 | STOP solution | 12ml | 4°C |
| ARG80128-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG80128 Human CCL2 / MCP1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human MCP-1 / CCL2 in serum, plasma and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | No significant cross-reactivity or interference with Human CXCL-16, MCP-2, MIP-1 alpha, MIP-1 beta, RANTES, GRO/MGSA, GRO gamma, GRO beta, IL-8; Mouse MIP-1 alpha, MIP-1 beta. |
| Target Name | CCL2 / MCP1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 8 pg/ml |
| Sample Type | Serum, plasma and cell culture supernatants. |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 100 µl |
| Precision | CV: less than10% |
| Alternate Names | MCP1; Monocyte chemotactic and activating factor; MCAF; Monocyte chemotactic protein 1; Monocyte secretory protein JE; HSMCR30; Small-inducible cytokine A2; HC11; SMC-CF; GDCF-2; SCYA2; C-C motif chemokine 2; Monocyte chemoattractant protein 1; MCP-1 |
Application Instructions
| Assay Time | 4 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CCL2 |
| Gene Full Name | chemokine (C-C motif) ligand 2 |
| Background | This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. [provided by RefSeq, Jul 2013] |
| Function | Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. [UniProt] |
| Highlight | Related products: MCP1 antibodies; MCP1 ELISA Kits; MCP1 Duos / Panels; Related news: HMGB1 in inflammation Inflammatory Cytokines A new role of fat – factory of circulating miRNAs Detecting MMPs and their non-ECM substrates New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Cancer kit; Cell Biology and Cellular Response kit; Immune System kit; Metabolism kit |
| PTM | Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant. |
Images (1) Click the Picture to Zoom In
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ARG80128 Human CCL2 / MCP1 ELISA Kit standard curve image
ARG80128 Human CCL2 / MCP1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
Specific References
Monoclonal enolase-1 blocking antibody ameliorates pulmonary inflammation and fibrosis
ELISA / Human / Culture supernatant
The Synergistic Inhibition of Coronavirus Replication and Induced Cytokine Production by Ciclesonide and the Tylophorine-Based Compound Dbq33b
ELISA / Human / Culture supernatant
Wnt5a promotes renal tubular inflammation in diabetic nephropathy by binding to CD146 through noncanonical Wnt signaling.
ELISA / Human / Cell culture supernatant
| Title | Download Link |
|---|---|
| ARG80128 Human CCL2 / MCP1 ELISA kit User's manual |
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