ARG81461
Human CCL13 / MCP4 ELISA Kit
Human CCL13 / MCP4 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81461-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81461-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81461-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81461-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81461-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81461-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81461-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81461-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81461-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81461-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81461-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81461 Human CCL13 / MCP4 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CCL13 / MCP4 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | CCL13 / MCP4 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 3.9 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 7.8 - 500 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.7% Inter-Assay CV: 6.8% |
| Alternate Names | SCYA13; C-C motif chemokine 13; Monocyte chemotactic protein 4; Small-inducible cytokine A13; CKb10; SCYL1; Monocyte chemoattractant protein 4; MCP-4; NCC-1; NCC1; CK-beta-10 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CCL13 |
| Gene Full Name | chemokine (C-C motif) ligand 13 |
| Background | This antimicrobial gene is one of several Cys-Cys (CC) cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. This chemokine plays a role in accumulation of leukocytes during inflammation. It may also be involved in the recruitment of monocytes into the arterial wall during artherosclerosis. [provided by RefSeq, Sep 2014] |
| Function | Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. Signals through CCR2B and CCR3 receptors. Plays a role in the accumulation of leukocytes at both sides of allergic and non-allergic inflammation. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. May play a role in the monocyte attraction in tissues chronically exposed to exogenous pathogens. [UniProt] |
| Highlight | Related products: CCL13 antibodies; CCL13 ELISA Kits; Related news: Detecting MMPs and their non-ECM substrates New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | One major form (form long), and two minor forms (short chain and medium chain) are produced by differential signal peptide cleavage. The medium chain is about 30-fold less active than the long chain. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81461 Human CCL13 / MCP4 ELISA Kit standard curve image
ARG81461 Human CCL13 / MCP4 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81461 Human CCL13 / MCP4 ELISA Kit User's manual |
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