ARG82794

Human 2-MET (Metanephrine + Normetanephrine) ELISA Kit

Human 2-MET (Metanephrine + Normetanephrine) ELISA Kit for ELISA and Human

Metabolism kit; Neuroscience kit; Signaling Transduction kit

Overview

Product Description ARG82794 Human 2-MET (Metanephrine + Normetanephrine) ELISA Kit is an Enzyme Immunoassay kit for the quantification of Metanephrine and Normetanephrine in Human urine.
Tested Reactivity Hu
Tested Application ELISA
Specificity Cross-Reactivity (Metanephrine):
Derivatized Metanephrine: 100%
Derivatized Normetanephrine: 0.15%
Derivatized 3-methoxytyramine: < 0.01%
Adrenaline: 3.3%
Noradrenaline: < 0.01%
Dopamine: < 0.01%
Vanillic mandelic acid, L-Dopa, Homovanillic acid, L-Tyrosin, Tyramin: < 0.01%

Cross-Reactivity (Normetanephrine):
Derivatized Metanephrine: 0.11%
Derivatized Normetanephrine: 100%
Derivatized 3-methoxytyramine: 0.19%
Adrenaline: < 0.01%
Noradrenaline: 0.64%
Dopamine: < 0.01%
Vanillic mandelic acid, L-Dopa, Homovanillic acid, L-Tyrosin, Tyramin: < 0.01%
Target Name 2-MET (Metanephrine + Normetanephrine)
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity Metanephrine: 10.5 ng/ml
Normetanephrine: 16.2 ng/ml
Sample Type Urine
Standard Range Metanephrine: 20 - 2000 ng/ml
Normetanephrine: 30 - 3000 ng/ml
Sample Volume 25 µl
Precision Metanephrine: Intra-Assay CV: 10.0% ; Inter-Assay CV: 16.3%
Normetanephrine: Intra-Assay CV: 11.0% ; Inter-Assay CV:15.0%

Application Instructions

Assay Time Hydrolysis and acylation 45 min; ELISA 1 hour

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

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Simplify the ELISA analysis by GainData
Research Area Metabolism kit; Neuroscience kit; Signaling Transduction kit

Images (2) Click the Picture to Zoom In

  • ARG82794 Human 2-MET (Metanephrine + Normetanephrine) ELISA Kit standard curve image

    ARG82794 Human 2-MET (Metanephrine + Normetanephrine) ELISA Kit results of a typical standard run with optical density reading at 450 nm.

  • ARG82794 Human 2-MET (Metanephrine + Normetanephrine) ELISA Kit standard curve image

    ARG82794 Human 2-MET (Metanephrine + Normetanephrine) ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG82794 Human 2-MET (Metanephrine + Normetanephrine) ELISA Kit User's manual download icon Download