ARG82031

Glycogen Branching Enzyme Assay Kit

Glycogen Branching Enzyme Assay Kit for Functional study and All species

Overview

Product Description ARG82031 Glycogen Branching Enzyme Assay Kit is a detection kit for the quantification of Glycogen Branching Enzyme Activity in tissue extracts, cell lysate and cell culture supernatants.
Tested Reactivity All
Tested Application FuncSt
Target Name Glycogen Branching Enzyme
Conjugation Note Read at 660 nm.
Detection Range 0.05 - 5 mmol/L
Sample Type Tissue extracts, cell lysate and cell culture supernatants.
Sample Volume 40 µl
Alternate Names 1,4-alpha-glucan-branching enzyme; GBE; Glycogen-branching enzyme; EC 2.4.1.18; APBD; GSD4; Brancher enzyme

Application Instructions

Assay Time ~ 35 min

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol GBE1
Gene Full Name glucan (1,4-alpha-), branching enzyme 1
Background The protein encoded by this gene is a glycogen branching enzyme that catalyzes the transfer of alpha-1,4-linked glucosyl units from the outer end of a glycogen chain to an alpha-1,6 position on the same or a neighboring glycogen chain. Branching of the chains is essential to increase the solubility of the glycogen molecule and, consequently, in reducing the osmotic pressure within cells. Highest level of this enzyme are found in liver and muscle. Mutations in this gene are associated with glycogen storage disease IV (also known as Andersen's disease). [provided by RefSeq, Jul 2008]
Function Required for sufficient glycogen accumulation. The alpha 1-6 branches of glycogen play an important role in increasing the solubility of the molecule and, consequently, in reducing the osmotic pressure within cells. [UniProt]

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  • ARG82031 Glycogen Branching Enzyme Assay Kit typical data demonstration image

    ARG82031 Glycogen Branching Enzyme Assay Kit results of a typical data with optical density reading at 660 nm.

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