ARG83048
Glutamine Assay Kit
Glutamine Assay Kit for Functional study and All species
Overview
| Product Description | ARG83048 Glutamine Assay Kit is a detection kit for the quantification of Glutamine in Urine, Serum, Plasma, Tissue extracts, Cell lysate, and Cell culture media. |
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| Tested Reactivity | All |
| Tested Application | FuncSt |
| Conjugation Note | Read at 420 nm. |
| Protein Full Name | Glutamine |
| Detection Range | 0.01 - 1 mmol/L |
| Sample Type | Urine, Serum, Plasma, Tissue extracts, Cell lysate, Cell culture and other biological fluids. |
| Sample Volume | 20 µl |
| Alternate Names | Glutamine, Gln, Q |
Application Instructions
| Assay Time | 90 min |
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Properties
| Form | 96 well |
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| Storage Instruction | Store kit reagents between 2-8°C. Immediately after use remaining reagents should be returned to cold storage (2-8°C). |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Background | Glutamine (symbol Gln or Q)is an α-amino acid that is used in the biosynthesis of proteins. Its side chain is similar to that of glutamic acid, except the carboxylic acid group is replaced by an amide. It is classified as a charge-neutral, polar amino acid. It is non-essential and conditionally essential in humans, meaning the body can usually synthesize sufficient amounts of it, but in some instances of stress, the body's demand for glutamine increases, and glutamine must be obtained from the diet.It is encoded by the codons CAA and CAG. In human blood, glutamine is the most abundant free amino acid. The dietary sources of glutamine include especially the protein-rich foods like beef, chicken, fish, dairy products, eggs, vegetables like beans, beets, cabbage, spinach, carrots, parsley, vegetable juices and also in wheat, papaya, Brussels sprouts, celery, kale and fermented foods like miso. |
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| Function | Glutamine plays a role in a variety of biochemical functions: *Protein synthesis, as any other of the 20 proteinogenic amino acids. *Lipid synthesis, especially by cancer cells.. *Regulation of acid-base balance in the kidney by producing ammonium. *Cellular energy, as a source, next to glucose. *Nitrogen donation for many anabolic processes, including the synthesis of purines. *Carbon donation, as a source, refilling the citric acid cycle. *Nontoxic transporter of ammonia in the blood circulation. *Integrity of healthy intestinal mucosa, though small randomized trials have shown no benefit in Crohn’s disease |
| Highlight | New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG83048 Glutamine Assay Kit standard curve image
ARG83048 Glutamine Assay Kit standard results of standard run with optical density reading at 450 nm
| Title | Download Link |
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| ARG83048 Glutamine Assay Kit User manual |
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