ARG30310
Endosome, Lysosome, Peroxisome Marker Antibody Panel (Catalase, Caveolin1, Clathrin heavy chain, LAMP1)
Publication3
Component
| Cat No | Component Name | Host clonality | Reactivity | Application | Package |
|---|---|---|---|---|---|
| ARG57976 | anti-Caveolin 1 antibody | Rabbit pAb | Hu, Ms | ICC/IF, IHC-P, WB | 50 μl |
| ARG63969 | anti-Catalase antibody | Goat pAb | Hu, Ms | IHC-P, WB | 50 μg |
| ARG52327 | anti-CD107a / LAMP1 antibody [5H6] | Mouse mAb | Hu, Bov | ICC/IF, WB | 50 μl |
| ARG53919 | anti-CLTC / Clathrin heavy chain antibody [BF-06] | Mouse mAb | Hu, Ms, Rat, Bov, Pig | ELISA, FACS, ICC/IF, IP, WB | 50 μg |
| ARG65350 | Goat anti-Mouse IgG antibody (HRP) | Goat pAb | Ms | ELISA, IHC-P, WB | 50 μl |
| ARG65351 | Goat anti-Rabbit IgG antibody (HRP) | Goat pAb | Rb | ELISA, IHC-P, WB | 50 μl |
Overview
| Product Description | Caveolin-1 (Cav-1) and Clathrin are markers for endosome. Cav-1 is a scaffolding protein within caveolar membranes in a transport vesicle. Clathrin is the major protein of the polyhedral coat of coated pits and vesicles and has a frunction in membrane invagination. The basic Clathrin oligomers are composed of three clathrin heavy chain (180 kDa) and three light chain (30 kDa) subunits and the process of polymerization is dynamically regulated by the light chains. Lysosome-associated membrane glycoprotein 1 (Lamp1) is a marker for lysosome while catalase is a marker for Peroxisome organelle. Lamp1 functions to present carbohydrates to selectins and Catalase converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Glenny et al. FEBS Lett 314: 45-48 (1992) Dodge et al. Genomics 11:174-178 (1991) Chen et al. J Proteome Res 8: 651-661 (2009) |
|---|---|
| Target Name | Endosome, Lysosome, Peroxisome Marker |
| Alternate Names | Endosome, Lysosome, Peroxisome Marker antibody; CD107a / LAMP1 antibody; CLTC / Clathrin heavy chain antibody; Caveolin 1 antibody; Catalase antibody |
Properties
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
|---|---|
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Images (11) Click the Picture to Zoom In
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ARG52327 anti-CD107a / LAMP1 antibody [5H6] WB image
Western blot: 30 µg of HeLa cell lysate stained with ARG52327 anti-CD107a / LAMP1 antibody [5H6] at 1:5000 dilution.
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ARG52327 anti-CD107a / LAMP1 antibody [5H6] ICC/IF image
Immunofluorescence: HeLa cells were treated with 50 µM of chloroquine, an inhibitor of autophagy, for 16 hours prior to staining. Cells stained with ARG52327 anti-CD107a / LAMP1 antibody [5H6] (red) at 1:500 dilution, and costained with ARG52468 anti-Vimentin antibody (green) at 1:10000 dilution. DAPI (blue) for nuclear staining.
Clone 5H6 reveals vesicular staining of LAMP1 protein accumulated in swollen lysosomes, while the Vimentin antibody specifically labels the intermediate filament network in these cells.
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ARG57976 anti-Caveolin 1 antibody ICC/IF image
Immunofluorescence: A431 cells stained with ARG57976 anti-Caveolin 1 antibody (green).
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ARG63969 anti-Catalase antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human spleen tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63969 anti-Catalase antibody at 2 µg/ml dilution followed by AP-staining.
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ARG57976 anti-Caveolin 1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse lung stained with ARG57976 anti-Caveolin 1 antibody.
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ARG63969 anti-Catalase antibody IHC-P image
Immunohistochemistry: Paraffin embedded Human Liver. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63969 anti-Catalase antibody at 2 µg/ml dilution followed by AP-staining.
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ARG63969 anti-Catalase antibody WB image
Western blot: 35 µg of Human kidney (A), Human liver (B), Mouse kidney (C) and Mouse liver (D) lysates (in RIPA buffer) stained with ARG63969 anti-Catalase antibody at 0.1 µg/ml dilution and incubated at RT for 1 hour.
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ARG53919 anti-CLTC / Clathrin heavy chain antibody [BF-06] ICC/IF image
Immunofluorescence: Human primary fibroblasts stained with ARG53919 anti-CLTC / Clathrin heavy chain antibody [BF-06] (green).
Actin cytoskeleton was stained with phalloidin (red) and cell nuclei stained with DAPI (blue). -
ARG53919 anti-CLTC / Clathrin heavy chain antibody [BF-06] ICC/IF image
Immunofluorescence: HeLa cells stained with ARG53919 anti-CLTC / Clathrin heavy chain antibody [BF-06] (green).
Actin cytoskeleton was stained with phalloidin (red) and cell nuclei stained with DAPI (blue). -
ARG57976 anti-Caveolin 1 antibody WB image
Western blot: A431 cell lysate stained with ARG57976 anti-Caveolin 1 antibody.
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ARG52327 anti-CD107a / LAMP1 antibody [5H6] WB image
Western blot: Cells were untreated or treated with 50 µM of chloroquine (CQ), an inhibitor of autophagy, for 24 hours. HeLa, HeLa + CQ, NIH/3T3 and NIH/3T3 + CQ (left to right) cell lysates stained with ARG52327 anti-CD107a / LAMP1 antibody [5H6] (green) at 1:10000 dilution. The smeared band between 75-120 kDa corresponds to variably glycosylated forms of the LAMP1 protein detected only in the Human cells, this antibody does not recognize the rodent LAMP1 homologue.
The same blot was stained with ARG10757 anti-Hsp 60 antibody (red) at 1:20000 dilution.
Specific References
Synaptic endocytosis in adult adipose stromal cell-derived neurons
ARG53919: ICC/IF / Human
Intercellular aspirin hand-over with dual therapies by liposome-loaded monocytes
ARG57976: ICC/IF / Mouse
KDF1 Promoted Proliferation, Migration and Invasion of Lung Adenocarcinoma Cells through Activating STAT3 and AKT Pathway
ARG65350: WB /
