ARG81357
Dengue virus NS1 ELISA Kit
Dengue virus NS1 ELISA Kit for ELISA and Dengue virus
Microbiology and Infectious Disease antibody
Publication7
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81357-001 | antibody-coated microplate | 12 X 8 strips | 4°C |
| ARG81357-002 | Standard (depends, e.g. 200 ng) | 1 vial | 4°C, store at -20 °C after reconstitution |
| ARG81357-003 | Standard reconstitution buffer | 1.1 mL | 4°C |
| ARG81357-004 | HRP-antibody Conjugate (200X) | 115 μL | 4°C |
| ARG81357-005 | Assay buffer | 12 mL | 4°C |
| ARG81357-006 | Detection antibody diluent buffer | 12 mL | 4°C |
| ARG81357-007 | 10X Wash buffer | 20 mL | 4°C |
| ARG81357-008 | TMB substrate | 12 mL (ready-to-use) | 4°C (Protect from light) |
| ARG81357-009 | STOP solution | 6 mL | 4°C |
| ARG81357-010 | Plate sealer | 1 strips | Room temperature |
Overview
| Product Description | ARG81357 Dengue virus NS1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Dengue virus NS1 in serum, plasma and cell culture supernatants. |
|---|---|
| Tested Reactivity | DEN |
| Tested Application | ELISA |
| Specificity | The ELISA kit reacts to DENV type 1-4 NS1 protein and it does not cross-react to ZIKV NS1 protein. |
| Target Name | Dengue virus NS1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | less than 0.5 ng/ml |
| Sample Type | Serum, plasma and cell culture supernatants. |
| Standard Range | 1.56 - 100 ng/ml |
| Sample Volume | 100 µl |
| Alternate Names | Dengue virus NS1 antibody; Dengue virus nonstructural glycoprotein NS1 antibody |
Application Instructions
| Assay Time | ~ 2.5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | DENV_gp1 |
|---|---|
| Gene Full Name | Dengue virus nonstructural protein 1 |
| Background | Dengue virus NS1 protein is a nonstructural protein which could be secreted and have been developed as diagnostic biomarker for early detection. There are several forms of NS1 including monomer, dimer, and hexamer during infection. Dimeric NS1 can be anchored to cell membranes with glycosyl-phosphatidylinositol (GPI). Hexameric NS1 can be secreted and detected in patients' blood samples (up to 50 μg/mL) or infected cell supernatants (various from ng/mL to μg/mL depend on serotypes and strains). Studies have shown that NS1 could interfere complement activity and prothrombin activation. In addition, NS1 could elicit antibodies which cross-react with host antigens including coagulation factors and molecules expressed in endothelial cells and platelets through molecular mimic. |
| Function | Dengue virus (DENV) non-structural protein 1 (NS1) is involved in virus replication and regulation of the innate immune response. Soluble and membrane-associated NS1 may activate human complement and induce host vascular leakage. This effect might explain the clinical manifestations of dengue hemorrhagic fever and dengue shock syndrome. [Uniprot] |
| Highlight | Related products: Dengue Virus antibodies; Dengue Virus ELISA Kits; Dengue Virus Duos / Panels; Related news: Best NS1 antibodies in the market Fighting fire with fire: Genetically-engineered mosquitoes as an alternative to fight diseases Tools for studying Dengue Virus Exploring Antiviral Immune Response New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Microbiology and Infectious Disease antibody |
Images (1) Click the Picture to Zoom In
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ARG81357 Dengue virus NS1 ELISA Kit standard curve image
ARG81357 Dengue virus NS1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
Specific References
Urinary dengue NS1 detection on Au-decorated ZnO nanowire platform
ELISA / Cell culture supernatants / Denguevirus
Elevated Interleukin-37 Associated with Dengue Viral Load in Patients with Dengue Fever
ELISA / Dengue virus / serum
Discrepant Activation Pattern of Inflammation and Pyroptosis Induced in Dermal Fibroblasts in Response to Dengue Virus Serotypes 1 and 2 and Nonstructural Protein 1
ELISA / Dengue virus / Cell culture supernatant
DENV NS1 and MMP-9 cooperate to induce vascular leakage by altering endothelial cell adhesion and tight junction.
ELISA / Dengue virus / Cell culture supernatant
Studies on the identification of antiviral lipid molecules against dengue virus using lipidomics.
ELISA / Dengue virus / Culture medium
High levels of serum hyaluronan is an early predictor of dengue warning signs and perturbs vascular integrity
ELISA / Dengue virus
Inhibition of dengue virus infection by 1-stearoyl-2-arachidonoyl-phosphatidylinositol in vitro.
ELISA / Dengue virus
| Title | Download Link |
|---|---|
| ARG81357 Dengue virus NS1 ELISA Kit User's manual |
 Download
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