ARG80972

Cholecystokinin 8, Octapeptide (non-Sulfated) ELISA Kit

Cholecystokinin 8, Octapeptide (non-Sulfated) ELISA Kit for ELISA and Human,Mouse,Rat

Metabolism kit; Signaling Transduction kit

Component

Cat No Component Name Package Temp
ARG80972-001 Secondary antibody-coated microplate 1 plate (96 well) 4°C
ARG80972-002 Primary antibody 1 vial 4°C
ARG80972-003 Standard peptide 1 vial 4°C
ARG80972-004 Biotinylated peptide 1 vial 4°C
ARG80972-005 SA-HRP concentrate 30 µl 4°C
ARG80972-006 20X Assay buffer 50 ml 4°C
ARG80972-007 Positive control 2 vials 4°C
ARG80972-008 Diluent buffer 23 ml 4°C
ARG80972-009 TMB substrate 12 ml 4°C (Protect from light)
ARG80972-010 STOP solution (2N HCl) 13 ml 4°C
ARG80972-011 Plate sealer 3 RT

Overview

Product Description ARG80972 Cholecystokinin 8, Octapeptide (non-Sulfated) ELISA kit is an Enzyme Immunoassay kit for the quantification of Human, Mouse and Rat Cholecystokinin 8, Octapeptide (non-Sulfated) in Blood collection.
Tested Reactivity Hu, Ms, Rat
Tested Application ELISA
Specificity Cross Reactivity:
CCK (26-33) (Non-Sulfated) (Human, Mouse, Rat): 100%
Big Gastrin-1 (Human): 100%
Caerulein: 100%
CCK-33 (Porcine): 100%
CCK (27-33): 100%
Gastrin-1 (Human): 100%
CCK-33 (Sulfated) (Human): 42.9%
CCK (30-33): 12.8%
CCK-33 (Non-Sulfated) (Human): 8%
CCK (26-33) (Sulfated): 3.9%
Pancreatic Polypeptide (Human): 0%
VIP (Human, Porcine, Rat): 0%
Target Name Cholecystokinin 8, Octapeptide (non-Sulfated)
A.A. Sequence DYMGWMDF-NH2
Conjugation HRP
Sensitivity 0.04 ng/ml
Detection Range 0.04 - 0.45 ng/ml
Sample Type Blood collection
Standard Range 0 - 100 ng/ml
Sample Volume 50 µl
Alternate Names CCK58; CCK12; CCK25; CCK7; CCK5; CCK18; CCK33; CCK8; CCK; 1-49; CCK39; Cholecystokinin

Application Instructions

Assay Time ~ 4 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12424 Mouse CCK

GeneID: 25298 Rat CCK

GeneID: 885 Human CCK

Gene Symbol CCK
Gene Full Name cholecystokinin
Background Cholecystokinin is a brain/gut peptide. In the gut, it induces the release of pancreatic enzymes and the contraction of the gallbladder. In the brain, its physiologic role is unclear. The cholecystokinin pro-hormone is processed by endo- and exo-proteolytic cleavages. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Mar 2010]
Function This peptide hormone induces gall bladder contraction and the release of pancreatic enzymes in the gut. Its function in the brain is not clear. Binding to CCK-A receptors stimulates amylase release from the pancreas, binding to CCK-B receptors stimulates gastric acid secretion. [UniProt]
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Research Area Metabolism kit; Signaling Transduction kit
PTM The precursor is cleaved by proteases to produce a number of active cholecystokinins.

Images (1) Click the Picture to Zoom In

  • ARG80972 Cholecystokinin 8, Octapeptide (non-Sulfated) ELISA Kit standard curve image

    ARG80972 Cholecystokinin 8, Octapeptide (non-Sulfated) ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG80972 Cholecystokinin 8, Octapeptide (non-Sulfated) ELISA Kit User manual download icon Download