ARG30321

Autophagy Antibody Panel

publication_link Publication17

Component

Cat No Component Name Host clonality Reactivity Application Package
ARG55799 anti-LC3B antibody Rabbit pAb Hu, Ms, Rat ICC/IF, IHC-P, WB 20 μl
ARG55040 anti-SQSTM1 / p62 antibody Rabbit pAb Hu, Ms, Rat ICC/IF, IHC-P, WB 20 μl
ARG58688 anti-Beclin 1 antibody Rabbit pAb Hu, Ms, Rat ICC/IF, IHC-P, WB 20 μl
ARG66330 anti-GAPDH antibody [SQab1878] Rabbit mAb AGMK, Bov, Chk, Hu, Ms, Pig, Rat, Xenopus laevis, Zfsh FACS, ICC/IF, IHC-P, IP, WB 20 μl
ARG65351 Goat anti-Rabbit IgG antibody (HRP) Goat pAb Rb ELISA, IHC-P, WB 50 μl

Overview

Product Description Autophagy Antibody Panel is an all-in-one solution to make autophagy research easy and economic. This antibody panel comprises antibodies against autophagy markers LC3B and SQSTM1 and antibody against the key regulator Beclin-1. It is ideal for not only autophagy detection but also investigation of autophagy regulation. Moreover, the most suitable loading control GAPDH antibody and the compatible secondary antibody are included in this panel. All the antibodies in this panel have excellent performance for multiple applications on multiple species.

Related news:
Keap1-Nrf2-ARE antibody panel is launched
New Autophagy antibody panel is released
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Target Name Autophagy
Alternate Names Autophagy antibody; SQSTM1 / p62 antibody; LC3B antibody; Beclin 1 antibody; GAPDH antibody

Properties

Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Full Name Antibody Panel for Autophagy
Highlight Related products:
Autophagy antibodies; Autophagy Duos / Panels;

Images (20) Click the Picture to Zoom In

  • ARG55040 anti-SQSTM1 / p62 antibody WB image

    Western blot: 20 µg of MCF7 cell lysate stained with ARG55040 anti-SQSTM1 / p62 antibody at 1:1000 dilution.

  • ARG55799 anti-LC3B antibody WB image (Customer's Feedback)

    Western blot: HeLa cells untreated or treated with Chloroquine. 20 µg of cell lysates stained with ARG55040 anti-SQSTM1 / p62 antibody and ARG55799 anti-LC3B antibody at 1:1000 dilution.

    An anti-GAPDH antibody used as a positive control.

  • ARG55040 anti-SQSTM1 / p62 antibody ICC/IF image

    Immunofluorescence: U2OS cells stained with ARG55040 anti-SQSTM1 / p62 antibody at 1:100 dilution.

  • ARG55799 anti-LC3B antibody ICC/IF image

    Immunofluorescence: NIH/3T3 cells treated with Chloroquine (50 μM, 37°C for 20 hours). Cells were stained with ARG55799 anti-LC3B antibody at 1:100 dilution.

  • ARG66330 anti-GAPDH antibody [SQab1878] ICC/IF image

    Immunofluorescence: HeLa cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for 30 min at RT. Cells were stained with ARG66330 anti-GAPDH antibody [SQab1878] (red) at 4°C. DAPI (blue) was used as the nuclear counter stain.

  • ARG58688 anti-Beclin 1 antibody ICC/IF image

    Immunofluorescence: HeLa cells stained with ARG58688 anti-Beclin 1 antibody (green). Nuclear staining (blue).

  • ARG66330 anti-GAPDH antibody [SQab1878] FACS image

    Flow Cytometry: HeLa cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The were stained with ARG66330 anti-GAPDH antibody [SQab1878] (red) in 1x PBS/1% BSA for 30 min at RT, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control. .

  • ARG55040 anti-SQSTM1 / p62 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat brain stained with ARG55040 anti-SQSTM1 / p62 antibody at 1:100 dilution.

  • ARG55799 anti-LC3B antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat kidney stained with ARG55799 anti-LC3B antibody at 1:100 dilution.

  • ARG66330 anti-GAPDH antibody [SQab1878] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded prostate cancer tissue stained with ARG66330 anti-GAPDH antibody [SQab1878]. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0.

  • ARG58688 anti-Beclin 1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human breast cancer tissue stained with ARG58688 anti-Beclin 1 antibody.

  • ARG66330 anti-GAPDH antibody [SQab1878] WB image

    Western blot: 2 µg of A431, MCF7, HCT-116, 293, HeLa, Raji and Jurkat cell lysates stained with ARG66330 anti-GAPDH antibody [SQab1878].

  • ARG58688 anti-Beclin 1 antibody WB image

    Western blot: HEK293 cell lysate stained with ARG58688 anti-Beclin 1 antibody.

  • ARG66330 anti-GAPDH antibody [SQab1878] IP image

    Immunoprecipitation: 0.1 mg of HeLa whole cell lysate immunoprecipitated and stained with ARG66330 anti-GAPDH antibody [SQab1878]. 1) ARG66330 IP in HeLa whole cell lysate, 2) PBS instead of ARG66330 in HeLa whole cell lysate, and 3) HeLa whole cell lysate, 2 µg (input).

  • ARG55040 anti-SQSTM1 / p62 antibody WB image

    Western blot: HeLa cells untreated or treated with Chloroquine. 20 µg of cell lysates stained with ARG55040 anti-SQSTM1 / p62 antibody at 1:1000 dilution.

  • ARG55799 anti-LC3B antibody WB image

    Western blot: 30 µg of HeLa untreated or treated with Chloroquine at 50 µM, 100 µM (18 hr). The bolts stained with ARG55799 anti-LC3B antibody at 1:1000 dilution.

  • ARG55040 anti-SQSTM1 / p62 antibody WB image

    Western blot: 30 µg of HeLa and A549 cell lysates stained with ARG55040 anti-SQSTM1 / p62 antibody at 1:500 dilution.

  • ARG66330 anti-GAPDH antibody [SQab1878] WB image

    Western blot: 2 µg of Mouse brain, Mouse heart, Mouse kidney, Mouse liver, Rat brain, Rat heart, Rat kidney and Rat liver lysates stained with ARG66330 anti-GAPDH antibody [SQab1878].

  • ARG66330 anti-GAPDH antibody [SQab1878] WB image

    Western blot: 2 µg of MDBK, Cos-7, Chicken heart, Pig heart, African clawed frog and Zebrafish lysates stained with ARG66330 anti-GAPDH antibody [SQab1878].

  • ARG55799 anti-LC3B antibody WB image

    Western blot: 30 µg of SW620, Mouse spleen, and Rat spleen lysates stained with ARG55799 anti-LC3B antibody at 1:1000 dilution.

Specific References

Rationale Design for Anchoring Pendant Groups of Zwitterionic Polymeric Medical Coatings

ARG65351: ELISA / 

Jia-Yin Chen et al.
Langmuir.,  (2024)

publication_link

 

hr_line

Discovery and mechanism of anti-hypertensive effect of a novel tripeptide (SYP) from medicinal fungus Ganoderma lingzhi

ARG66330: WB / Human

Qiang Wu et al.
Pre-Print,  (2024)

publication_link

 

hr_line

High expression of circulating exosomal PD-L1 contributes to immune escape of hepatocellular carcinoma and immune clearance of chronic hepatitis B

ARG65351: WB / 

Xiaoqing Lin et al.
Aging (Albany NY),  (2024)

publication_link

 

hr_line

Ginsenoside Rb1 alleviated concanavalin A-induced hepatocyte pyroptosis by activating mitophagy

ARG55040: WB / Human, Mouse

Qian Zhang et al.
Food Funct.,  (2023)

publication_link

 

hr_line

Alcohol induces hepatocytes necroptosis through the LC3/RIPK1/RIPK3 pathway

ARG55040: WB / Mouse

Yuelin Chen et al.
Food Chem Toxicol.,  (2023)

publication_link

 

hr_line

Cardiac-Specific Gene TNNI3 Acts as a Potential Oncogene for Papillary Renal Cell Carcinoma

ARG66330: WB / Human

Biao Cai et al.
,  (2023)

publication_link

 

hr_line

Poloxamer 407 and Hyaluronic Acid Thermosensitive Hydrogel-Encapsulated Ginsenoside Rg3 to Promote Skin Wound Healing

ARG55040: WB / Mouse

Xiaojuan Peng et al.
Front Bioeng Biotechnol.,  (2022)

publication_link

 

hr_line

Class IIa histone deacetylase inhibition ameliorates acute kidney injury by suppressing renal tubular cell apoptosis and enhancing autophagy and proliferation

ARG65614: WB / Mouse

Jialu Li et al.
Front Pharmacol.,  (2022)

publication_link

 

hr_line

Integrinβ3 Mediates the Protective Effect of Soluble Receptor for Advanced Glycation End-products During Myocardial Ischemia/Reperfusion

ARG55799: WB / Mouse

Xuejie Han et al.
Research Square,  (2021)

publication_link

 

hr_line

MicroRNA-145 Delay Plaque Erosion by Down-Regulating TGFβr Ii and Suppressing Autophagy of Vascular Smooth Muscle Cells.

ARG55040: WB, IHC-P, ICC/IF / Human

Yunjuan Jiao et al.
Preprint.,  (2021)

publication_link

 

hr_line

Necroptotic virotherapy of oncolytic alphavirus M1 cooperated with Doxorubicin displays promising therapeutic efficacy in TNBC

ARG66330: WB / Human

Jiayu Zhang et al.
Oncogene,  (2021)

publication_link

 

hr_line

Curcumin and tetrahydrocurcumin induce cell death in Ara-C-resistant acute myeloid leukemia.

ARG55040: WB / Human

Tseng Yu-Hsin et al.
Phytother Res.,  (2019)

publication_link

 

hr_line

Bone Morphogenetic Protein 15 Knockdown Inhibits Porcine Ovarian Follicular Development and Ovulation.

ARG55799: IHC-P / Pig

Qin Yufeng et al.
Front Cell Dev Biol.,  (2019)

publication_link

 

hr_line

Role of Autophagy in Acquired Cholesteatoma.

ARG55799: WB, IHC-P; ARG65614: WB / Human

Li Quancheng et al.
Otol Neurotol.,  (2019)

publication_link

 

hr_line

Redd1 protects against post‑infarction cardiac dysfunction by targeting apoptosis and autophagy.

ARG55799: WB, ICC/IF / Mouse

Huang Pian-Pian et al.
Int J Mol Med.,  (2019)

publication_link

 

hr_line

Essential role of Bone morphogenetic protein 15 in porcine ovarian and follicular development and ovulation.

ARG55799: IHC-P / Pig

Qin Yufeng et al.
Preprint.,  (2019)

publication_link

 

hr_line

新生大鼠早期感染致脑白质损伤自噬相关蛋白的表达

ARG65614: WB / Rat

孟 孟 et al.
中国免疫学杂志,  (2017)

publication_link

 

hr_line