ARG30321
Autophagy Antibody Panel
Component
Cat No | Component Name | Host clonality | Reactivity | Application | Package |
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ARG55799 | anti-LC3B antibody | Rabbit pAb | Hu, Ms, Rat | ICC/IF, IHC-P, WB | 20 μl |
ARG55040 | anti-SQSTM1 / p62 antibody | Rabbit pAb | Hu, Ms, Rat | ICC/IF, IHC-P, WB | 20 μl |
ARG58688 | anti-Beclin 1 antibody | Rabbit pAb | Hu, Ms, Rat | ICC/IF, IHC-P, WB | 20 μl |
ARG66330 | anti-GAPDH antibody [SQab1878] | Rabbit mAb | AGMK, Bov, Chk, Hu, Ms, Pig, Rat, Xenopus laevis, Zfsh | FACS, ICC/IF, IHC-P, IP, WB | 20 μl |
ARG65351 | Goat anti-Rabbit IgG antibody (HRP) | Goat pAb | Rb | ELISA, IHC-P, WB | 50 μl |
Overview
Product Description | Autophagy Antibody Panel is an all-in-one solution to make autophagy research easy and economic. This antibody panel comprises antibodies against autophagy markers LC3B and SQSTM1 and antibody against the key regulator Beclin-1. It is ideal for not only autophagy detection but also investigation of autophagy regulation. Moreover, the most suitable loading control GAPDH antibody and the compatible secondary antibody are included in this panel. All the antibodies in this panel have excellent performance for multiple applications on multiple species. Related news: Keap1-Nrf2-ARE antibody panel is launched New Autophagy antibody panel is released Therapeutic strategies against PDAC |
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Target Name | Autophagy |
Alternate Names | Autophagy antibody; SQSTM1 / p62 antibody; LC3B antibody; Beclin 1 antibody; GAPDH antibody |
Properties
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
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Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Gene Full Name | Antibody Panel for Autophagy |
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Highlight | Related products: Autophagy antibodies; Autophagy Duos / Panels; |
Images (20) Click the Picture to Zoom In
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ARG55040 anti-SQSTM1 / p62 antibody WB image
Western blot: 20 µg of MCF7 cell lysate stained with ARG55040 anti-SQSTM1 / p62 antibody at 1:1000 dilution.
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ARG55799 anti-LC3B antibody WB image (Customer's Feedback)
Western blot: HeLa cells untreated or treated with Chloroquine. 20 µg of cell lysates stained with ARG55040 anti-SQSTM1 / p62 antibody and ARG55799 anti-LC3B antibody at 1:1000 dilution.
An anti-GAPDH antibody used as a positive control.
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ARG55040 anti-SQSTM1 / p62 antibody ICC/IF image
Immunofluorescence: U2OS cells stained with ARG55040 anti-SQSTM1 / p62 antibody at 1:100 dilution.
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ARG55799 anti-LC3B antibody ICC/IF image
Immunofluorescence: NIH/3T3 cells treated with Chloroquine (50 μM, 37°C for 20 hours). Cells were stained with ARG55799 anti-LC3B antibody at 1:100 dilution.
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ARG66330 anti-GAPDH antibody [SQab1878] ICC/IF image
Immunofluorescence: HeLa cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for 30 min at RT. Cells were stained with ARG66330 anti-GAPDH antibody [SQab1878] (red) at 4°C. DAPI (blue) was used as the nuclear counter stain.
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ARG58688 anti-Beclin 1 antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG58688 anti-Beclin 1 antibody (green). Nuclear staining (blue).
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ARG66330 anti-GAPDH antibody [SQab1878] FACS image
Flow Cytometry: HeLa cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The were stained with ARG66330 anti-GAPDH antibody [SQab1878] (red) in 1x PBS/1% BSA for 30 min at RT, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control. .
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ARG55040 anti-SQSTM1 / p62 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat brain stained with ARG55040 anti-SQSTM1 / p62 antibody at 1:100 dilution.
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ARG55799 anti-LC3B antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat kidney stained with ARG55799 anti-LC3B antibody at 1:100 dilution.
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ARG66330 anti-GAPDH antibody [SQab1878] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded prostate cancer tissue stained with ARG66330 anti-GAPDH antibody [SQab1878]. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0.
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ARG58688 anti-Beclin 1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human breast cancer tissue stained with ARG58688 anti-Beclin 1 antibody.
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ARG66330 anti-GAPDH antibody [SQab1878] WB image
Western blot: 2 µg of A431, MCF7, HCT-116, 293, HeLa, Raji and Jurkat cell lysates stained with ARG66330 anti-GAPDH antibody [SQab1878].
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ARG58688 anti-Beclin 1 antibody WB image
Western blot: HEK293 cell lysate stained with ARG58688 anti-Beclin 1 antibody.
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ARG66330 anti-GAPDH antibody [SQab1878] IP image
Immunoprecipitation: 0.1 mg of HeLa whole cell lysate immunoprecipitated and stained with ARG66330 anti-GAPDH antibody [SQab1878]. 1) ARG66330 IP in HeLa whole cell lysate, 2) PBS instead of ARG66330 in HeLa whole cell lysate, and 3) HeLa whole cell lysate, 2 µg (input).
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ARG55040 anti-SQSTM1 / p62 antibody WB image
Western blot: HeLa cells untreated or treated with Chloroquine. 20 µg of cell lysates stained with ARG55040 anti-SQSTM1 / p62 antibody at 1:1000 dilution.
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ARG55799 anti-LC3B antibody WB image
Western blot: 30 µg of HeLa untreated or treated with Chloroquine at 50 µM, 100 µM (18 hr). The bolts stained with ARG55799 anti-LC3B antibody at 1:1000 dilution.
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ARG55040 anti-SQSTM1 / p62 antibody WB image
Western blot: 30 µg of HeLa and A549 cell lysates stained with ARG55040 anti-SQSTM1 / p62 antibody at 1:500 dilution.
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ARG66330 anti-GAPDH antibody [SQab1878] WB image
Western blot: 2 µg of Mouse brain, Mouse heart, Mouse kidney, Mouse liver, Rat brain, Rat heart, Rat kidney and Rat liver lysates stained with ARG66330 anti-GAPDH antibody [SQab1878].
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ARG66330 anti-GAPDH antibody [SQab1878] WB image
Western blot: 2 µg of MDBK, Cos-7, Chicken heart, Pig heart, African clawed frog and Zebrafish lysates stained with ARG66330 anti-GAPDH antibody [SQab1878].
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ARG55799 anti-LC3B antibody WB image
Western blot: 30 µg of SW620, Mouse spleen, and Rat spleen lysates stained with ARG55799 anti-LC3B antibody at 1:1000 dilution.
Specific References